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DSMZ

DSMZ 630 . MODIFIED THERMUS 162 MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Rhodothermus marinus DSM 4253

* Modification: Plus 1% NaCl

Growth conditions: 65 °C

Main sol. 630 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 2.5 g 2.5 -
Tryptone 2.5 g 2.5 35.172
Agar 28.0 g 28 83.252
Nitrilotriacetic acid 100.0 mg 0.1 0.523
CaSO4 x 2 H2O 40.0 mg 0.04 0.232
MgCl2 x 6 H2O 200.0 mg 0.2 0.984
Fe(III) citrate
(0.01 M) 		0.5 ml 0.001 0.005
Trace element solution 0.5 ml - -
Phosphate buffer 100.0 ml - -
NaCl* 10.0 g/l - -
Distilled water 900.0 ml - -
1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. Autoclave the phosphate buffer separately and then add to the medium.

Phosphate buffer #

1000
Solution occurs in 3 other media (first defined in Medium 630).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 5.44 g 5.44 39.975
Na2HPO4 x 12 H2O 43.00 g 43 302.904
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.8 g 12.8 66.967
FeCl2 x 4 H2O 1.0 g 1 5.03
MnCl2 x 4 H2O 0.5 g 0.5 2.526
CoCl2 x 4 H2O 0.3 g 0.3 2.311
CuCl2 x 2 H2O 50.0 mg 0.05 0.293
Na2MoO4 x 2 H2O 50.0 mg 0.05 0.207
H3BO3 20.0 mg 0.02 0.323
NiCl2 x 6 H2O 20.0 mg 0.02 0.154
Distilled water 1000.0 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 8 - 9 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 14
Strains with modifications: 3
Rhodothermus marinus DSM 4253

  10.0 g/l NaCl

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Agar 28 83.252
NaCl 10 171.116
Na2HPO4 x 12 H2O 4.3 30.29
Tryptone 2.5 35.172
Yeast extract 2.5 -
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Cultivation metadata from 14 strains BacDive

pH 6 - 8 1 strain
phyla 3 14 strains
oxygen aerobe 1 strain
temperature thermophilic 14 strains