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DSMZ

DSMZ 607 . M13 VERRUCOMICROBIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Rubinisphaera brasiliensis DSM 5305

This strain uses the standard medium without modifications.

Growth conditions: 7-14 days aerobic 30 °C

Main sol. 607 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.25 g 0.25 -
Yeast extract 0.25 g 0.25 -
Glucose 0.25 g 0.25 1.388
Vitamin solution 10.00 ml - -
Hutner's salts 20.00 ml - -
Tris-HCl buffer
(0.1M, pH 7.5) 		50.00 ml - 5
Artifical sea water 250.00 ml - -
Distilled water 670.00 ml - -
1 Autoclave at 121°C for 15 min.

Artifical sea water #

1000
Solution occurs in 2 other media (first defined in Medium 607).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.477 g 23.477 401.728
Na2SO4 3.917 g 3.917 27.577
MgCl2 x 6 H2O 4.981 g 4.981 24.5
CaCl2 1.102 g 1.102 9.93
NaHCO3 192.000 mg 0.192 2.286
KCl 664.000 mg 0.664 8.907
KBr 6.000 mg 0.006 0.05
H3BO3 26.000 mg 0.026 0.42
SrCl2 24.000 mg 0.024 0.151
NaF 3.000 mg 0.003 0.071
Distilled water 1000.000 ml - -

Vitamin solution #

1000
Solution occurs in 2 other media (first defined in Medium 603).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
Biotin 2.0 mg 0.002 0.008
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Calcium pantothenate 5.0 mg 0.005 0.01
Folic acid 2.0 mg 0.002 0.005
Riboflavin 5.0 mg 0.005 0.013
Nicotinamide 5.0 mg 0.005 0.041
Distilled water 1000.0 ml - -
1 Filter-sterilized

Hutner's salts #

1000
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 7
Rubinisphaera brasiliensis DSM 5305

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 5.869 100.432
MgCl2 x 6 H2O 1.245 6.125
Na2SO4 0.979 6.894
MgSO4 x 7 H2O 0.594 2.41
CaCl2 0.276 2.482
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Cultivation metadata from 7 strains BacDive

phyla 3 7 strains
nutrition chemoheterotroph 1 strain
oxygen obligate aerobe 6 strains
oxygen aerobe 1 strain
temperature mesophilic 6 strains