This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone | 0.25 | g | 0.25 | - | |
Yeast extract | 0.25 | g | 0.25 | - | |
Glucose | 0.25 | g | 0.25 | 1.388 | |
Vitamin solution | 10.00 | ml | - | - | |
Hutner's salts | 20.00 | ml | - | - | |
Tris-HCl buffer
(0.1M, pH 7.5) |
50.00 | ml | - | 5 | |
Artifical sea water | 250.00 | ml | - | - | |
Distilled water | 670.00 | ml | - | - | |
1 Autoclave at 121°C for 15 min. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 23.477 | g | 23.477 | 401.728 |
Na2SO4 | 3.917 | g | 3.917 | 27.577 |
MgCl2 x 6 H2O | 4.981 | g | 4.981 | 24.5 |
CaCl2 | 1.102 | g | 1.102 | 9.93 |
NaHCO3 | 192.000 | mg | 0.192 | 2.286 |
KCl | 664.000 | mg | 0.664 | 8.907 |
KBr | 6.000 | mg | 0.006 | 0.05 |
H3BO3 | 26.000 | mg | 0.026 | 0.42 |
SrCl2 | 24.000 | mg | 0.024 | 0.151 |
NaF | 3.000 | mg | 0.003 | 0.071 |
Distilled water | 1000.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 | |
Biotin | 2.0 | mg | 0.002 | 0.008 | |
Thiamine-HCl x 2 H2O | 5.0 | mg | 0.005 | 0.013 | |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 | |
Folic acid | 2.0 | mg | 0.002 | 0.005 | |
Riboflavin | 5.0 | mg | 0.005 | 0.013 | |
Nicotinamide | 5.0 | mg | 0.005 | 0.041 | |
Distilled water | 1000.0 | ml | - | - | |
1 Filter-sterilized |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
"Metals 44" | 50.000 | ml | - | - | |
Distilled water | 950.000 | ml | - | - | |
1 Dissolve the nitrilotriacetic acid. | |||||
2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
3 Dissolve other salts separately and combine. | |||||
4 Adjust pH to 6.8 with NaOH or H2SO4. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. |
Last modified: | 15.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 7 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 5.869 | 100.432 |
MgCl2 x 6 H2O | 1.245 | 6.125 |
Na2SO4 | 0.979 | 6.894 |
MgSO4 x 7 H2O | 0.594 | 2.41 |
CaCl2 | 0.276 | 2.482 |
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