548 .
BENNETT'S AGAR
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Equipment needed:
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Beef extract | 1 | g | ||
|
|
Glucose | 10 | g | ||
|
|
N-Z amine
(enzymatic digest of casein) |
2 | g | ||
|
|
Yeast extract | 1 | g | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.3 with NaOH. | |||||
| 2 Modified Bennett's agar can also be used. In it, glucose is replaced by glycerol (10.0 g) and N-Z amine by Bacto casein (2.0 g, Difco). | |||||