545c
545c .
TRYPTONE SOYA BROTH (ANAEROB)
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. 545c #
ml
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 17.0 | g | 17 | - | |
| Soy peptone | 3.0 | g | 3 | - | |
| D(+)-Glucose | 2.5 | g | 2.5 | 13.877 | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.3 | 1.708 | |
| Na-thioglycolate | 0.3 | g | 0.3 | 2.629 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and thioglycolate), boil medium for 1 minute, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and thioglycolate, then adjust the pH to 8.0. Dispense medium under the same gas atmosphere in culture vessels and autoclave at 121ºC for 15 minutes. Check and adjust the pH of the complete medium to 8.0, if necessary. | |||||
Metadata
| Last modified: | 27.03.24 |
| Source: | MediaDive: DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 8.0 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Casein peptone | 17 | - |
| NaCl | 5 | 85.558 |
| Soy peptone | 3 | - |
| K2HPO4 | 2.5 | 14.353 |
| D(+)-Glucose | 2.5 | 13.877 |
| Show more | ||
