473 .
MEDIUM WITH EDTA AS CARBON SOURCE
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Equipment needed:
Autoclave
Final pH:
7.5 - 7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 1.400 | g | ||
|
|
KH2PO4 | 0.272 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.020 | g | ||
|
|
MgSO4 x 7 H2O | 0.490 | g | ||
|
|
Ferric ammonium citrate | 0.080 | g | ||
|
|
Na2-EDTA | 0.200 | g | ||
|
|
Agar | 15.000 | g | ||
|
|
Trace element solution SL-6 | 5.000 | ml | ||
|
|
Distilled water | 1000.000 | ml | ||
|
|
Vitamin solution | 5.000 | ml | ||
| 1 Autoclave phosphate buffer separately. pH should be 7.5 - 7.6. Add 5.0 ml sterile stock solution (see medium 462). | |||||
DSM 6780 Vitamin solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 0.08 | g |
|
|
Folic acid | 0.08 | g |
|
|
Thiamine HCl | 0.08 | g |
|
|
Distilled water | 100.00 | ml |
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |
Vitamin solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
p-Aminobenzoate | 1.0 | mg |
|
|
Biotin | 0.2 | mg |
|
|
Nicotinic acid | 2.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 1.0 | mg |
|
|
Calcium pantothenate | 0.5 | mg |
|
|
Pyridoxamine | 5.0 | mg |
|
|
Vitamin B12 | 2.0 | mg |
|
|
Distilled water | 100.0 | ml |