# 473: MEDIUM WITH EDTA AS CARBON SOURCE


## Main sol. 473

1.4	g	K2HPO4	
0.272	g	KH2PO4	
0.02	g	Ca(NO3)2 x 4 H2O	
0.49	g	MgSO4 x 7 H2O	
0.08	g	Ferric ammonium citrate	
0.2	g	Na2-EDTA	
15	g	Agar	
5	ml	**Trace element solution SL-6**	
1000	ml	Distilled water	
5	ml	**Vitamin solution**	

1. Autoclave phosphate buffer separately. pH should be 7.5 - 7.6. Add 5.0 ml sterile stock solution (see medium 462).


## DSM 6780 Vitamin solution

0.08	g	Biotin	
0.08	g	Folic acid	
0.08	g	Thiamine HCl	
100	ml	Distilled water	


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Vitamin solution

1	mg	p-Aminobenzoate	
0.2	mg	Biotin	
2	mg	Nicotinic acid	
1	mg	Thiamine-HCl x 2 H2O	
0.5	mg	Calcium pantothenate	
5	mg	Pyridoxamine	
2	mg	Vitamin B12	
100	ml	Distilled water