MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 452 . GLUCOSE MEDIUM (NAKAYAMA)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Sporolactobacillus laevolacticus DSM 6548

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 452 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 10 g 10 55.506
Peptone 10 g 10 -
Yeast extract 15 g 15 -
Agar 10 g 10 29.733
Distilled water 980 ml - -
Solution A 10 ml - -
Solution B 10 ml - -
Solution C 1 ml - -
1 Autoclave all solutions separately, final pH is 6.8.

Solution A #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.5 g 5 36.742
K2HPO4 0.5 g 5 28.707
Distilled water 100.0 ml - -

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 3.00 g 30 121.716
NaCl 0.10 g 1 17.112
MnSO4 x 5 H2O 0.10 g 1 4.148
CuSO4 x 5 H2O 0.01 g 0.1 0.401
Distilled water 100.00 ml - -

Solution C #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
Na3-citrate 2.0 g 20 77.499
Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 14
Sporolactobacillus laevolacticus DSM 6548

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 15 -
Glucose 10 55.506
Agar 10 29.733
Peptone 10 -
MgSO4 x 7 H2O 0.3 1.217
Show more
CSV
JSON

Cultivation metadata from 14 strains BacDive

phyla 1 14 strains
temperature mesophilic 14 strains