376 .
AN1 MEDIUM
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Equipment needed:
Anaerobic workbench
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase
(BBL) |
10.0 | g | ||
|
|
K2HPO4 | 1.5 | g | ||
|
|
NaCl | 2.5 | g | ||
|
|
Na-thioglycolate | 1.0 | g | ||
|
|
Sulfur
(powdered) |
8.0 | g | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
| 2 Prepare the medium anaerobically under 100% nitrogen. Sterilize thioglycolate and sulfur separately. Sterilize the sulfur by steaming for 3 h on each of three successive days. | |||||