376
376 .
AN1 MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Thermococcus zilligii DSM 2770
This strain uses the standard medium without modifications.
Growth conditions: 75 °C
Main sol. 376 #
ml
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
10.0 | g | 10 | - | |
| K2HPO4 | 1.5 | g | 1.5 | 8.612 | |
| NaCl | 2.5 | g | 2.5 | 42.779 | |
| Na-thioglycolate | 1.0 | g | 1 | 8.764 | |
|
Sulfur
(powdered) |
8.0 | g | 8 | 249.454 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
| 2 Prepare the medium anaerobically under 100% nitrogen. Sterilize thioglycolate and sulfur separately. Sterilize the sulfur by steaming for 3 h on each of three successive days. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Equipment needed: | Anaerobic workbench |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thermococcus zilligii DSM 2770
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase | 10 | - |
| Sulfur | 8 | 249.454 |
| NaCl | 2.5 | 42.779 |
| K2HPO4 | 1.5 | 8.612 |
| Na-thioglycolate | 1 | 8.764 |
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