Main sol. 376
Internal Solution-ID: S716
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
10.0 | g | 10 | - | |
| K2HPO4 | 1.5 | g | 1.5 | 8.612 | |
| NaCl | 2.5 | g | 2.5 | 42.779 | |
| Na-thioglycolate | 1.0 | g | 1 | 8.764 | |
|
Sulfur
(powdered) |
8.0 | g | 8 | 249.454 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
| 2 Prepare the medium anaerobically under 100% nitrogen. Sterilize thioglycolate and sulfur separately. Sterilize the sulfur by steaming for 3 h on each of three successive days. | |||||
Media with this solution
| 376 | AN1 MEDIUM |