371 .
NATRONOBACTERIA MEDIUM
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Equipment needed:
Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.00 | g | ||
|
|
KCl | 1.00 | g | ||
|
|
NH4Cl | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.24 | g | ||
|
|
CaSO4 x 2 H2O | 0.17 | g | ||
|
|
Trace element solution SL-10 | 1.00 | ml | ||
|
|
Agar , if necessary | 20.00 | g | ||
|
|
NaCl | 200.00 | g | ||
|
|
Na2-glutamate | 1.00 | g | ||
|
|
Yeast extract
(Fisher BioReagents BP1422) |
5.00 | g | ||
|
|
Casamino acids | 5.00 | g | ||
|
|
Na2CO3 | 5.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Add distilled water to give a final volume of 1000 ml. | |||||
| 2 Adjust pH to 6.5 before autoclaving! | |||||
| 3 Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5. | |||||
| 4 If agar medium is prepared, heat and dissolve agar before adding sodium chloride. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||