# 371: NATRONOBACTERIA MEDIUM


## Main sol. 371

1	g	KH2PO4	
1	g	KCl	
1	g	NH4Cl	
0.24	g	MgSO4 x 7 H2O	
0.17	g	CaSO4 x 2 H2O	
1	ml	**Trace element solution SL-10**	
20	g	Agar, if necessary	
200	g	NaCl	
1	g	Na2-glutamate	
5	g	Yeast extract (Fisher BioReagents BP1422)	
5	g	Casamino acids	
5	g	Na2CO3	
1000	ml	Distilled water	

1. Add distilled water to give a final volume of 1000 ml.
2. Adjust pH to 6.5 before autoclaving!
3. Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5.
4. If agar medium is prepared, heat and dissolve agar before adding sodium chloride.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.