Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
CaCl2 x 2 H2O | 0.13 | g | 0.13 | 0.884 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
Trace element solution | 10.00 | ml | - | - | |
Wolin's vitamin solution | 5.00 | ml | - | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Yeast extract | 0.50 | g | 0.5 | - | |
Glycine | 1.50 | g | 1.5 | 19.981 | |
KH2PO4 | 3.00 | g | 3 | 22.045 | |
Na2HPO4 | 5.80 | g | 5.8 | 40.857 | |
NaHCO3 | 0.50 | g | 0.5 | 5.952 | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.2 - 7.4 with NaOH. | |||||
2 Prepare the medium anaerobically under 100% nitrogen. Prepare concentrated solutions each of yeast extract, glycine, phosphates, NaHCO3 and Na2S separately and sterilize under nitrogen by autoclaving. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
CoCl2 x 6 H2O | 0.17 | g | 0.17 | 1.309 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
CuCl2 | 0.02 | g | 0.02 | 0.149 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
NaCl | 1.00 | g | 1 | 17.112 | |
Na2SeO3 x 5 H2O | 0.03 | g | 0.03 | 0.114 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 - 7.4 |
Equipment needed: | Autoclave |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2HPO4 | 5.8 | 40.857 |
KH2PO4 | 3 | 22.045 |
Glycine | 1.5 | 19.981 |
NH4Cl | 1 | 18.695 |
NaHCO3 | 0.5 | 5.952 |
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