Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.13 | g | 0.13 | 0.884 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
| Trace element solution | 10.00 | ml | - | - | |
| Wolin's vitamin solution | 5.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| Glycine | 1.50 | g | 1.5 | 19.981 | |
| KH2PO4 | 3.00 | g | 3 | 22.045 | |
| Na2HPO4 | 5.80 | g | 5.8 | 40.857 | |
| NaHCO3 | 0.50 | g | 0.5 | 5.952 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.2 - 7.4 with NaOH. | |||||
| 2 Prepare the medium anaerobically under 100% nitrogen. Prepare concentrated solutions each of yeast extract, glycine, phosphates, NaHCO3 and Na2S separately and sterilize under nitrogen by autoclaving. | |||||
Media with this solution
| 331 | ACETOBACTEROIDES GLYCINOPHILUS MEDIUM |