289
289 .
RUMINICLOSTRIDIUM PAPYROSOLVENS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 289 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea water
(filtered) |
200.00 | ml | - | - | |
| Mineral salt solution | 150.00 | ml | - | - | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| Yeast extract | 0.60 | g | 0.6 | - | |
| K2HPO4 | 1.65 | g | 1.65 | 9.473 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Cellobiose | 5.00 | g | 5 | 14.607 | |
|
Cellulose
, Lens tissue or MN 301
optional |
5.00 | g | 5 | - | |
| Distilled water | 650.00 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and cellobiose) and sparge medium with 100% N2 gas for 30 - 45 m in to make it anoxic. Add cysteine, adjust pH to 7.2 and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose from an anoxic stock solution sterilized by filtration. | |||||
| 2 Note: Some strains can be adapted to cellulose as substrate using 5.0 g/l cellulose (Lens tissue, OXOID; or cellulose powder MN 301, MACHEREY-NAGEL). If necessary adjust pH of completed medium to 7.2. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| (NH4)2SO4 | 6.0 | g | 6 | 45.406 |
| NaCl | 6.0 | g | 6 | 102.669 |
| MgSO4 x 7 H2O | 1.2 | g | 1.2 | 4.869 |
| CaCl2 x 2 H2O | 0.8 | g | 0.8 | 5.442 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Cellobiose | 5 | 14.607 |
|
Cellulose optional |
5 | - |
| K2HPO4 | 1.65 | 9.473 |
| NH4Cl | 1 | 18.695 |
| NaCl | 0.9 | 15.4 |
| Show more | ||
