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DSMZ

DSMZ 27 . RHODOSPIRILLACEAE MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Rhodomicrobium vannielii DSM 23296

This strain uses the standard medium without modifications.

Growth conditions: 7-14 days anaerobic in light 28 °C

Main sol. 27 #

ml
Equipment needed: Tungsten lamp Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.30 g 0.3 -
Disodium succinate 1.00 g 1 6.171
Ammonium acetate 0.50 g 0.5 6.487
Fe(III) citrate
(0.1% in H2O) 		5.00 ml 0.005 -
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.40 g 0.4 1.623
NaCl 0.40 g 0.4 6.845
NH4Cl 0.40 g 0.4 7.478
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Vitamin B12
(10 mg in 100 ml H2O) 		0.40 ml - -
Trace element solution SL-6 1.00 ml - -
L-Cysteine HCl 0.30 g 0.3 1.903
Resazurin
(0,1%) 		0.50 ml 0.005 0.022
Distilled water 1000.00 ml - -
1 Adjust pH to 6.8.
2 Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples.
3 Incubate in the light using a tungsten lamp.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Tungsten lamp
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Associated strains

All strains for this medium: 89
Strains with modifications: 22
Rhodomicrobium vannielii DSM 23296

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
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Cultivation metadata from 89 strains BacDive

pH 6 - 8 10 strains
pH 8 - 10 8 strains
pH 4 - 6 2 strains
phyla 1 99 strains
nutrition copiotroph|diazotroph 1 strain
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