MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 27 . RHODOSPIRILLACEAE MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Blastochloris viridis DSM 133

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days 28 °C

Main sol. 27 #

ml
Equipment needed: Tungsten lamp Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.30 g 0.3 -
Disodium succinate 1.00 g 1 6.171
Ammonium acetate 0.50 g 0.5 6.487
Fe(III) citrate
(0.1% in H2O) 		5.00 ml 0.005 -
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.40 g 0.4 1.623
NaCl 0.40 g 0.4 6.845
NH4Cl 0.40 g 0.4 7.478
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Vitamin B12
(10 mg in 100 ml H2O) 		0.40 ml - -
Trace element solution SL-6 1.00 ml - -
L-Cysteine HCl 0.30 g 0.3 1.903
Resazurin
(0,1%) 		0.50 ml 0.005 0.022
Distilled water 1000.00 ml - -
1 Adjust pH to 6.8.
2 Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples.
3 Incubate in the light using a tungsten lamp.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Tungsten lamp
Edit in Medium builder

Associated strains

All strains for this medium: 89
Strains with modifications: 22
Blastochloris viridis DSM 133

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
Show more
CSV
JSON

Cultivation metadata from 89 strains BacDive

pH 6 - 8 10 strains
pH 8 - 10 8 strains
pH 4 - 6 2 strains
phyla 1 99 strains
nutrition copiotroph|diazotroph 1 strain
Show more