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POLYANGIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
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Medium for Sorangium cellulosum DSM 2276
This strain uses the standard medium without modifications.
Growth conditions: 26 °C
Main sol. 223 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KNO3 | 1.00 | g | 1 | 9.891 | |
| K2HPO4 | 1.00 | g | 1 | 5.741 | |
| MgSO4 x 7 H2O | 0.30 | g | 0.3 | 1.217 | |
| CaCl2 x 2 H2O | 0.13 | g | 0.13 | 0.884 | |
| FeCl3 x 6 H2O | 32.00 | mg | 0.032 | 0.118 | |
| Agar | 10.00 | g | 10 | 29.733 | |
| Tap water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.2 (use pH indicator-paper). | |||||
| 2 Aseptically add one or more sterile strips of filter paper to slants or plates. Inoculate the filter papers with a small (5 mm x 5mm) agar cube or a piece of filter paper overgrown with the strain. | |||||
Metadata
| Last modified: | 08.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Sorangium cellulosum DSM 2276
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 10 | 29.733 |
| KNO3 | 1 | 9.891 |
| K2HPO4 | 1 | 5.741 |
| MgSO4 x 7 H2O | 0.3 | 1.217 |
| CaCl2 x 2 H2O | 0.13 | 0.884 |
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