Main sol. 223
Internal Solution-ID: S483
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KNO3 | 1.00 | g | 1 | 9.891 | |
| K2HPO4 | 1.00 | g | 1 | 5.741 | |
| MgSO4 x 7 H2O | 0.30 | g | 0.3 | 1.217 | |
| CaCl2 x 2 H2O | 0.13 | g | 0.13 | 0.884 | |
| FeCl3 x 6 H2O | 32.00 | mg | 0.032 | 0.118 | |
| Agar | 10.00 | g | 10 | 29.733 | |
| Tap water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.2 (use pH indicator-paper). | |||||
| 2 Aseptically add one or more sterile strips of filter paper to slants or plates. Inoculate the filter papers with a small (5 mm x 5mm) agar cube or a piece of filter paper overgrown with the strain. | |||||
Media with this solution
| 223 | POLYANGIUM MEDIUM |