220a .
REACTIVATION WITH TRYPTONE SOYA BROTH
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Selected strain: Diaphorobacter nitroreducens DSM 15985
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
220. CASO AGAR (Merck 105458) , alternative | 1000 | ml | ||
|
|
545. TRYPTONE SOYA BROTH (TSB) , alternative | 1000 | ml | ||
| 1 Rehydrate and grow lyophilized cells from the ampoule in liquid tryptone soya broth (see medium 545). Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
220. CASO AGAR (Merck 105458) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 15 | g | ||
|
|
Soy peptone | 5 | g | ||
|
|
NaCl | 5 | g | ||
|
|
Agar | 15 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131). | |||||
545. TRYPTONE SOYA BROTH (TSB) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 17.0 | g | ||
|
|
Soy peptone | 3.0 | g | ||
|
|
D(+)-Glucose | 2.5 | g | ||
|
|
NaCl | 5.0 | g | ||
|
|
K2HPO4 | 2.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||