220a
220a .
REACTIVATION WITH TRYPTONE SOYA BROTH
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Diaphorobacter nitroreducens DSM 15985
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Main sol. 220a #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 220. CASO AGAR (Merck 105458) , alternative | 1000 | ml | - | - | |
| 545. TRYPTONE SOYA BROTH (TSB) , alternative | 1000 | ml | - | - | |
| 1 Rehydrate and grow lyophilized cells from the ampoule in liquid tryptone soya broth (see medium 545). Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 15 | g | 15 | - | |
| Soy peptone | 5 | g | 5 | - | |
| NaCl | 5 | g | 5 | 85.558 | |
| Agar | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131). | |||||
Solution occurs in 4
other media (first defined in Medium 54a).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 17.0 | g | 17 | - | |
| Soy peptone | 3.0 | g | 3 | - | |
| D(+)-Glucose | 2.5 | g | 2.5 | 13.877 | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 58 |
Diaphorobacter nitroreducens DSM 15985
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Casein peptone | 17 | - |
| Agar | 15 | 44.599 |
| NaCl | 5 | 85.558 |
| Soy peptone | 3 | - |
| K2HPO4 | 2.5 | 14.353 |
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