{"medium":{"id":"220a","name":"REACTIVATION WITH TRYPTONE SOYA BROTH","complex_medium":"yes","min_pH":7.3,"max_pH":7.3,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220a.pdf"},"solutions":[{"id":478,"name":"Main sol. 220a","volume":1000,"recipe":[{"recipe No.":1,"solution":"220. CASO AGAR (Merck 105458)","solution_id":479,"condition":"alternative","amount":1000,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"545. TRYPTONE SOYA BROTH (TSB)","solution_id":72,"condition":"alternative","amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Rehydrate and grow lyophilized cells from the ampoule in liquid tryptone soya broth (see medium 545). Subsequent subculturing may be carried out in liquid medium or on agar plates."}],"main solution":"yes"},{"id":479,"name":"220. CASO AGAR (Merck 105458)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Casein peptone","compound_id":21,"amount":15,"unit":"g","g_l":15,"optional":"no"},{"recipe No.":2,"compound":"Soy peptone","compound_id":75,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":3,"compound":"NaCl","compound_id":43,"amount":5,"unit":"g","g_l":5,"mmol_l":85.5578,"optional":"no"},{"recipe No.":4,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","g_l":15,"mmol_l":44.5991,"optional":"no"},{"recipe No.":5,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131)."}],"main solution":"no"},{"id":72,"name":"545. TRYPTONE SOYA BROTH (TSB)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Casein peptone","compound_id":21,"amount":17,"unit":"g","g_l":17,"optional":"no"},{"recipe No.":2,"compound":"Soy peptone","compound_id":75,"amount":3,"unit":"g","g_l":3,"optional":"no"},{"recipe No.":3,"compound":"D(+)-Glucose","compound_id":94,"amount":2.5,"unit":"g","g_l":2.5,"mmol_l":13.8766,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":5,"unit":"g","g_l":5,"mmol_l":85.5578,"optional":"no"},{"recipe No.":5,"compound":"K2HPO4","compound_id":10,"amount":2.5,"unit":"g","g_l":2.5,"mmol_l":14.3533,"optional":"no"},{"recipe No.":6,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.3."},{"step":"The same as Caso Bouillon (Merck or Oxoid, Germany)."}],"main solution":"no"}]}