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THERMOPROTEUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Thermoproteus tenax DSM 2078
This strain uses the standard medium without modifications.
Growth conditions: 85 °C
Main sol. 185 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.264 | g | 0.264 | 1.998 | |
| FeSO4 x 7 H2O | 0.556 | g | 0.556 | 2 | |
| MgSO4 x 7 H2O | 0.492 | g | 0.492 | 1.996 | |
| CaSO4 x 2 H2O | 0.344 | g | 0.344 | 1.998 | |
| KH2PO4 | 0.014 | g | 0.014 | 0.103 | |
| Resazurin | 1.000 | mg | 1.0e-3 | 0.004 | |
| Trace elements solution | 1.000 | ml | - | - | |
| Yeast extract | 0.200 | g | 0.2 | - | |
|
Starch
(soluble) |
5.000 | g | 5 | - | |
|
Sulfur
(powdered) |
10.000 | g | 10 | 311.818 | |
| Na2S x 9 H2O | 0.500 | g | 0.5 | 2.082 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Prepare medium (without starch, sodium sulfide, sulfur) anaerobically under N2 gas atmosphere. Adjust pH to 5.5 with H2SO4 before sterilization. Distribute the medium into tubes containing the appropriate amount of sulfur powder. Sterilize medium by heating for 3 h at 90 - 100°C on three subsequent days. Before use, add to the medium starch and sodium sulfide. | |||||
| 2 Medium pH is 5.5. | |||||
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaF | 840 | mg | 0.84 | 20.006 |
| MnCl2 x 4 H2O | 180 | mg | 0.18 | 0.91 |
| Na2B4O7 x 10 H2O | 450 | mg | 0.45 | - |
| ZnSO4 x 7 H2O | 22 | mg | 0.022 | 0.077 |
| CuCl2 x 2 H2O | 5 | mg | 0.005 | 0.029 |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 |
| CoSO4 x 7 H2O | 1 | mg | 1.0e-3 | 0.006 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 5.5 |
| Equipment needed: | Anaerobic workbench |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thermoproteus tenax DSM 2078
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 10 | 311.818 |
| Starch | 5 | - |
| FeSO4 x 7 H2O | 0.556 | 2 |
| Na2S x 9 H2O | 0.5 | 2.082 |
| MgSO4 x 7 H2O | 0.492 | 1.996 |
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