MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 185

Download this solution as

CSV JSON PDF

Internal Solution-ID: S311

Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.264 g 0.264 1.998
FeSO4 x 7 H2O 0.556 g 0.556 2
MgSO4 x 7 H2O 0.492 g 0.492 1.996
CaSO4 x 2 H2O 0.344 g 0.344 1.998
KH2PO4 0.014 g 0.014 0.103
Resazurin 1.000 mg 1.0e-3 0.004
Trace elements solution 1.000 ml - -
Yeast extract 0.200 g 0.2 -
Starch
(soluble) 		5.000 g 5 -
Sulfur
(powdered) 		10.000 g 10 311.818
Na2S x 9 H2O 0.500 g 0.5 2.082
Distilled water 1000.000 ml - -
1 Prepare medium (without starch, sodium sulfide, sulfur) anaerobically under N2 gas atmosphere. Adjust pH to 5.5 with H2SO4 before sterilization. Distribute the medium into tubes containing the appropriate amount of sulfur powder. Sterilize medium by heating for 3 h at 90 - 100°C on three subsequent days. Before use, add to the medium starch and sodium sulfide.
2 Medium pH is 5.5.
185 THERMOPROTEUS MEDIUM