1825
1825 . MDM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.

×

Main sol. 1825 #

ml

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Salt mix 10x	100	ml	-	-
Amino acids mix 5x	200	ml	-	-
Trace salt mix 100x	10	ml	-	-
Iron stock solution 2.0 g/L	3	ml	-	-
Vitamine mixture	5	ml	-	-
Glucose stock 20%	10	ml	-	-
1 Make up to 1000 ml with sterile distilled water.
2 anaerobize with nitrogen, can be stored anaerobically for a limited time

Salt mix 10x #

1000

Solution occurs in 1 other media (first defined in Medium 1757).

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂HPO₄	50	g/l	50	352.214
NaH₂PO₄ x H₂O	20	g/l	20	166.699
KH₂PO₄	9	g/l	9	66.135
NaCl	9	g/l	9	154.004
1 autoclave

Trace salt mix 100x #

1000

Solution occurs in 1 other media (first defined in Medium 1757).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
(NH₄)₂SO₄	4.000	g/l	4	30.271
CaCl₂ x 2 H₂O	2.600	g/l	2.6	17.685
MgCl₂ x 6 H₂O	2.000	g/l	2	9.838
MnCl₂ x 4 H₂O	1.000	g/l	1	5.053
CoCl₂ x 6 H₂O	0.100	g/l	0.1	0.77
NaHSeO₃ , almost no effect on growth in CDMM, more important in MDM	0.015	g/l	0.015	-
1 steril filtration

Iron stock solution 2.0 g/L #

100

Solution occurs in 1 other media (first defined in Medium 1757).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
FeSO₄ x 7 H₂O (Sigma Aldrich!)	2	g/l	2	7.194
1 steril filtration, store in aliquots at -20°C

Vitamine mixture #

500

Solution occurs in 1 other media (first defined in Medium 1757).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
D-Biotin	0.06	g/l	0.06	0.246
Calcium D-(+)-pantothenate	0.20	g/l	0.2	0.839
Pyridoxine	0.20	g/l	0.2	1.182
1 steril filtration, store aliquots at -20°C

Glucose stock 20% #

500

Solution occurs in 1 other media (first defined in Medium 1757).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
alpha-D-Glucose , not required but growth enhancing	200	g/l	200	1110.15
1 steril filtration

Amino acids mix 5x #

1000

Equipment needed: Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
L-Cysteine	10.0	g	10	82.536
L-Tryptophan	0.5	g	0.5	2.448
L-Leucine	2.0	g	2	15.247
L-Valine	1.5	g	1.5	12.804
L-Proline	10.0	g	10	86.858
L-Isoleucine	1.5	g	1.5	11.436
L-Methionine	1.0	g	1	6.702
1 Adjust pH to 6.0-7.0.
2 Filtrate.
3 Store at -20°C

Metadata

Last modified:	31.08.23
Source:	MediaDive: DSMZ
Taxonomic range:	Bacteria
Medium type:	Defined medium
Final pH:	6.0 - 7.0
Equipment needed:	Autoclave, Filter
Reference:	doi.org/10.1186/s12866-015-0614-2
Description: Minimal medium for Clostridioides difficile. It is a minimal medium with only required amino acids, growth can be strongly enhanced by the addition of L-threonine. Adapted from Karlsson S, Burman LG, Akerlund T. Suppression of toxin production in Clostridium difficile VPI 10463 by amino acids. Microbiology. 1999;145(Pt 7):1683–93.

Edit in Medium builder

Associated strains

All strains for this medium:

2

Compound	Conc. [g/L]	Conc. [mM]
Na₂HPO₄	5	35.221
L-Proline	2	17.372
NaH₂PO₄ x H₂O	2	16.67
L-Cysteine	2	16.507
alpha-D-Glucose	2	11.102
NaCl	0.9	15.4
KH₂PO₄	0.9	6.614
L-Leucine	0.4	3.049
L-Valine	0.3	2.561
L-Isoleucine	0.3	2.287
L-Methionine	0.2	1.34
L-Tryptophan	0.1	0.49
(NH₄)₂SO₄	0.04	0.303
CaCl₂ x 2 H₂O	0.026	0.177
MgCl₂ x 6 H₂O	0.02	0.098
MnCl₂ x 4 H₂O	0.01	0.051
FeSO₄ x 7 H₂O	0.006	0.022
CoCl₂ x 6 H₂O	1.0e-3	0.008
Pyridoxine	1.0e-3	0.006
Calcium D-(+)-pantothenate	1.0e-3	0.004
D-Biotin	3.0e-4	0.001
NaHSeO₃	1.5e-4	-
Show more Show less

CSV

JSON

phyla	1	2 strains
oxygen	anaerobe	2 strains
temperature	mesophilic	2 strains

Have some cookies!

We are using cookies to improve the user experience. We save the following information: user preferences for language, accessibility, help features and medium favourites. Unfortunately, you cannot use this site if you don't agree.