1757
1757 .
CDMM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Salt mix 10x | 100 | ml | - | - | |
| CasAA mix 5x | 200 | ml | - | - | |
| Trace salt mix 100x | 10 | ml | - | - | |
| Iron stock solution 2.0 g/L | 3 | ml | - | - | |
| Vitamine mixture | 5 | ml | - | - | |
| Glucose stock 20% | 10 | ml | - | - | |
| 1 fill to 1 Liter with H2O (autoclaved) | |||||
| 2 anaerobize with nitrogen, can be stored anaerobically | |||||
Solution occurs in 1
other media (first defined in Medium 1757).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 | 50 | g/l | 50 | 352.214 | |
| NaH2PO4 x H2O | 20 | g/l | 20 | 166.699 | |
| KH2PO4 | 9 | g/l | 9 | 66.135 | |
| NaCl | 9 | g/l | 9 | 154.004 | |
| 1 autoclave | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casamino acids | 50.0 | g/l | 50 | - | |
| L-Cysteine | 2.5 | g/l | 2.5 | 20.634 | |
| L-Tryptophan | 0.5 | g/l | 0.5 | 2.448 | |
| 1 pH should be between 6-7, may depend on the casamino acid and cysteine lot | |||||
| 2 steril filtration, store as batches at -20°C | |||||
Solution occurs in 1
other media (first defined in Medium 1757).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 4.000 | g/l | 4 | 30.271 | |
| CaCl2 x 2 H2O | 2.600 | g/l | 2.6 | 17.685 | |
| MgCl2 x 6 H2O | 2.000 | g/l | 2 | 9.838 | |
| MnCl2 x 4 H2O | 1.000 | g/l | 1 | 5.053 | |
| CoCl2 x 6 H2O | 0.100 | g/l | 0.1 | 0.77 | |
| NaHSeO3 , almost no effect on growth in CDMM, more important in MDM | 0.015 | g/l | 0.015 | - | |
| 1 steril filtration | |||||
Solution occurs in 1
other media (first defined in Medium 1757).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
FeSO4 x 7 H2O
(Sigma Aldrich!) |
2 | g/l | 2 | 7.194 | |
| 1 steril filtration, store in aliquots at -20°C | |||||
Solution occurs in 1
other media (first defined in Medium 1757).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| D-Biotin | 0.06 | g/l | 0.06 | 0.246 | |
| Calcium D-(+)-pantothenate | 0.20 | g/l | 0.2 | 0.839 | |
| Pyridoxine | 0.20 | g/l | 0.2 | 1.182 | |
| 1 steril filtration, store aliquots at -20°C | |||||
Solution occurs in 1
other media (first defined in Medium 1757).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| alpha-D-Glucose , not required but growth enhancing | 200 | g/l | 200 | 1110.15 | |
| 1 steril filtration | |||||
Metadata
| Last modified: | 31.08.23 |
| Source: | MediaDive: DSMZ |
| Synonyms: | Clostridioides difficile defined medium |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Reference: | doi.org/10.1186/s12866-015-0614-2 |
|
Description:
Clostridioides difficile defined medium, often referred to as minimal medium but the casamino acids contain amino acids that are not required in a minimal medium. Batch of casamino acids has a major influence of maximal growth. Adapted from Cartman ST, Minton NP. A mariner-based transposon system for in vivo random mutagenesis of Clostridium difficile. Appl Environ Microbiol. 2010;76:1103–9. |
|
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 4 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Casamino acids | 10 | - |
| Na2HPO4 | 5 | 35.221 |
| NaH2PO4 x H2O | 2 | 16.67 |
| alpha-D-Glucose | 2 | 11.102 |
| NaCl | 0.9 | 15.4 |
| Show more | ||
