1723a
1723a .
Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1723a #
ml
| 1 Prepare 1l artificial sea water in milliQ water using: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Artificial Sea Salt
, ~ 18 mS/cm = ~ 12 psu at 25°C
(hw Marinemix 21010, Wiegandt GmbH, Sterkenhofweg 13, 47807 Krefeld, Germany) |
12.4 | g/l | 12.4 | - | |
| 2 Then add the following components: | |||||
|
NaNO3
(75 g/l stock solution) |
1.0 | ml/l | 0.075 | 0.882 | |
|
NaH2PO4 x H2O
(5 g/l stock solution) |
1.0 | ml/l | 0.005 | 0.042 | |
| f/2 Trace Metal Mix | 1.0 | ml | - | - | |
| 3 Autoclave for 20 min at 121°C. | |||||
| 4 After cooling, add the following filter sterilized (0.2 µm) components to complete the medium: | |||||
| Vitamin 3 Mix | 1.0 | ml | - | - | |
| 5 The strain is cultivated in 25 cm2 plastic tissue culture flasks with filter screw caps (T25, distributor: TPP, order number: 90026). The T25 flasks are routinely filled with 20 ml of culture. Each T25 culture is supplemented with a sterilized wheat grain as carbon source for ambient bacteria from the sampling site. | |||||
|
Wheat Grains
(sterilized by autoclaving at 121°C for 20 min) |
trace | - | - | ||
Solution occurs in 1
other media (first defined in Medium 1723).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 4.16 | g/l | 4.16 | 12.373 |
| FeCl3 x 6 H2O | 3.15 | g/l | 3.15 | 11.654 |
|
MnCl2 x 4 H2O
(0.4 g/100 ml stock solution) |
45.00 | ml/l | - | - |
|
ZnSO4 x 7 H2O
(1.2 g/l stock solution) |
18.30 | ml/l | 0.022 | 0.076 |
|
CoCl2 x 6 H2O
(0.6 g/100 ml stock solution) |
1.70 | ml/l | - | - |
|
CuSO4 x 5 H2O
(1.25 g/100 ml stock solution) |
0.80 | ml/l | - | - |
|
Na2 MoO4 x 2 H2O
(0.27 g/100 ml stock solution) |
2.20 | ml/l | - | - |
Solution occurs in 1
other media (first defined in Medium 1723).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Thiamine HCl
(1 g/100 ml stock solution) |
1.00 | ml | - | - |
|
D-Biotin
(0.05 g/100 ml stock solution) |
0.10 | ml | - | - |
|
Cyanocobalamin
(Vitamin B12; 0.2 g/l stock solution) |
0.25 | ml | - | - |
Metadata
| Last modified: | 16.08.23 |
| Source: | MediaDive: DSMZ |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |