Main sol. 1723a
Internal Solution-ID: S5983
| 1 Prepare 1l artificial sea water in milliQ water using: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Artificial Sea Salt
, ~ 18 mS/cm = ~ 12 psu at 25°C
(hw Marinemix 21010, Wiegandt GmbH, Sterkenhofweg 13, 47807 Krefeld, Germany) |
12.4 | g/l | 12.4 | - | |
| 2 Then add the following components: | |||||
|
NaNO3
(75 g/l stock solution) |
1.0 | ml/l | 0.075 | 0.882 | |
|
NaH2PO4 x H2O
(5 g/l stock solution) |
1.0 | ml/l | 0.005 | 0.042 | |
| f/2 Trace Metal Mix | 1.0 | ml | - | - | |
| 3 Autoclave for 20 min at 121°C. | |||||
| 4 After cooling, add the following filter sterilized (0.2 µm) components to complete the medium: | |||||
| Vitamin 3 Mix | 1.0 | ml | - | - | |
| 5 The strain is cultivated in 25 cm2 plastic tissue culture flasks with filter screw caps (T25, distributor: TPP, order number: 90026). The T25 flasks are routinely filled with 20 ml of culture. Each T25 culture is supplemented with a sterilized wheat grain as carbon source for ambient bacteria from the sampling site. | |||||
|
Wheat Grains
(sterilized by autoclaving at 121°C for 20 min) |
trace | - | - | ||
Media with this solution
| 1723a | Enriched Artificial Seawater Medium f/2 suppl. with Wheat Grains |