167 .
SPOROCYTOPHAGA MEDIUM
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Equipment needed:
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaNO3 | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 6.00 | mg | ||
|
|
KH2PO4 | 0.14 | g | ||
|
|
K2HPO4 | 1.20 | g | ||
|
|
Yeast extract | 0.02 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
Agar
optional |
15.00 | g/l | ||
| 1 Adjust pH to 7.2. | |||||
| 2 Place a strip of sterile cellulose filter paper onto an agar plate. Alternatively, place a strip of sterile cellulose filter paper into a culture tube containing 5 ml of medium, thereby leaving 1 - 2 cm of the strip outside of the medium. Inoculate the strain onto the cellulose filter paper. The strain will grow on the cellulose paper only. | |||||