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DSMZ

DSMZ 166 . HYPHOMICROBIUM STRAIN X MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Hyphomicrobium denitrificans DSM 1869

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 166 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.00 g 1 7.568
MgSO4 x 7 H2O 0.20 g 0.2 0.811
NaH2PO4 x H2O 0.50 g 0.5 4.167
K2HPO4 1.55 g 1.55 8.899
Trace element solution (Vishniac & Santer, 1957) 0.20 ml - -
Methylamine hydrochloride 3.40 g 3.4 50.355
Agar 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2. For liquid medium use only 0.7 g of methylamine hydrochloride per liter.

Trace element solution (Vishniac & Santer, 1957) #

1000
Solution occurs in 4 other media (first defined in Medium 69).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
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Associated strains

All strains for this medium: 1
Hyphomicrobium denitrificans DSM 1869

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Methylamine hydrochloride 3.4 50.355
K2HPO4 1.55 8.899
(NH4)2SO4 1 7.568
NaH2PO4 x H2O 0.5 4.167
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain