1592
1592 .
Cyanobacteria Medium BG11-
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Anabaena sp. DSM 101377
* Modification: Standing culture, 16 hours at 160 lux illumination, 8-hours dark period. Transfer interval of 4 weeks
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 18 °C
Main sol. 1592 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Mg(SO4) x 7 H2O
(7.5 g/l stock solution) |
10 | ml | - | - | |
|
CaCl2 x 2 H2O
(3.6 g/l stock solution) |
10 | ml | - | - | |
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
10 | ml | - | - | |
|
Na-EDTA x 2 H2O
(0.1 g/l stock solution) |
10 | ml | - | - | |
| Trace metal mix | 1 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 After cooling, add the following filter sterilized components. | |||||
| BG11-Mix | 2 | ml | - | - | |
|
Vitamin B12
(0.02 g/l stock solution) |
1 | ml | - | - | |
Solution occurs in 2
other media (first defined in Medium 1592).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| H3BO3 | 2.90 | g | 2.9 | 46.895 | |
| MnCl2 x 4 H2O | 1.81 | g | 1.81 | 9.146 | |
| ZnSO4 x 7 H2O | 0.22 | g | 0.22 | 0.765 | |
| Na2MoO4 x 2 H2O | 0.39 | g | 0.39 | 1.612 | |
|
CuSO4 x 5 H2O
(0.8 g/100 ml stock solution) |
10.00 | ml | - | - | |
|
Co(NO3)2 x 6 H2O
(0.5 g/100 ml stock solution) |
10.00 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
Solution occurs in 3
other media (first defined in Medium 1592).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 x 3 H2O | 20 | g | 20 | 114.826 | |
| Na2CO3 | 10 | g | 10 | 94.35 | |
| Fe(NH4)citrate | 3 | g | 3 | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
Metadata
| Last modified: | 09.11.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Light
| Intensity | 160 lux lux |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| K2HPO4 x 3 H2O | 0.04 | 0.23 |
| Na2CO3 | 0.02 | 0.189 |
| Fe(NH4)citrate | 0.006 | - |
| H3BO3 | 0.003 | 0.047 |
| MnCl2 x 4 H2O | 0.002 | 0.009 |
| Show more | ||
Cultivation metadata from 28 strains
| phyla | 1 | 21 strains |
| temperature | psychrophilic | 20 strains |