{"medium":{"id":1592,"name":"Cyanobacteria Medium BG11-","complex_medium":"no","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1592.pdf"},"solutions":[{"id":5518,"name":"Main sol. 1592","volume":1000,"recipe":[{"recipe No.":1,"compound":"Mg(SO4) x 7 H2O","compound_id":2154,"attribute":"7.5 g/l stock solution","amount":10,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"CaCl2 x 2 H2O","compound_id":7,"attribute":"3.6 g/l stock solution","amount":10,"unit":"ml","optional":"no"},{"recipe No.":3,"compound":"Na3-citrate x 2 H2O","compound_id":2155,"attribute":"0.6 g/l stock solution","amount":10,"unit":"ml","optional":"no"},{"recipe No.":4,"compound":"Na-EDTA x 2 H2O","compound_id":890,"attribute":"0.1 g/l stock solution","amount":10,"unit":"ml","optional":"no"},{"recipe No.":5,"compound":"K2HPO4 x 3 H2O","compound_id":244,"amount":2,"unit":"ml","optional":"no"},{"recipe No.":6,"compound":"Na2CO3","compound_id":32,"amount":2,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Fe(NH4)citrate","compound_id":1203,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":8,"solution":"Trace metal mix","solution_id":5734,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":12,"compound":"Vitamin B12","compound_id":18,"attribute":"0.02 g/l stock solution","amount":1,"unit":"ml","optional":"no"}],"steps":[{"recipe No.":9,"step":"Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water."},{"recipe No.":10,"step":"Autoclave at 121 \u00b0C for 20 min."},{"recipe No.":11,"step":"After cooling, add the following filter sterilized components."}],"equipment":{"5144":"Autoclave"},"main solution":"yes"},{"id":5734,"name":"Trace metal mix","volume":1000,"recipe":[{"recipe No.":1,"compound":"H3BO3","compound_id":37,"amount":2.86,"unit":"g","g_l":2.9,"mmol_l":46.8952,"optional":"no"},{"recipe No.":2,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":1.81,"unit":"g","g_l":1.81,"mmol_l":9.1458,"optional":"no"},{"recipe No.":3,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.22,"unit":"g","g_l":0.22,"mmol_l":0.765055,"optional":"no"},{"recipe No.":4,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.39,"unit":"g","g_l":0.39,"mmol_l":1.6119,"optional":"no"},{"recipe No.":5,"compound":"CuSO4 x 5 H2O","compound_id":121,"attribute":"8.0 g/l stock solution","amount":10,"unit":"ml","optional":"no"},{"recipe No.":6,"compound":"Co(NO3)2 x 6 H2O","compound_id":541,"attribute":"5.0 g/l stock solution","amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water."}],"main solution":"no"}]}