Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Mg(SO4) x 7 H2O
(7.5 g/l stock solution) |
10 | ml | - | - | |
|
CaCl2 x 2 H2O
(3.6 g/l stock solution) |
10 | ml | - | - | |
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
10 | ml | - | - | |
|
Na-EDTA x 2 H2O
(0.1 g/l stock solution) |
10 | ml | - | - | |
| K2HPO4 x 3 H2O | 2 | ml | - | - | |
| Na2CO3 | 2 | ml | - | - | |
| Fe(NH4)citrate | 1 | ml | - | - | |
| Trace metal mix | 1 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 After cooling, add the following filter sterilized components. | |||||
|
Vitamin B12
(0.02 g/l stock solution) |
1 | ml | - | - | |
Media with this solution
| 1592 | Cyanobacteria Medium BG11- |