1565
1565 .
ALKALIPHILUS TRANSVAALENSIS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Alkaliphilus transvaalensis DSM 29504
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Main sol. 1565 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 100.00 | ml | - | - | |
|
Yeast extract
(BD Bacto) |
2.00 | g | 2 | - | |
|
Tryptone
(BD Bacto) |
2.00 | g | 2 | - | |
| Na2-tartrate x 2 H2O | 1.00 | g | 1 | - | |
| Na3-citrate | 1.00 | g | 1 | 3.875 | |
| NaNO3 | 0.25 | g | 0.25 | 2.941 | |
| (NH4)2SO4 | 0.25 | g | 0.25 | 1.892 | |
| Ferric citrate monohydrate | 0.20 | g | 0.2 | 0.761 | |
| Na2SO4 | 1.00 | g | 1 | 7.04 | |
| Na2CO3 | 1.25 | g | 1.25 | 11.794 | |
| NaHCO3 | 2.50 | g | 2.5 | 29.759 | |
| Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, bicarbonate, and thiosulfate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and bicarbonate, adjust pH to 10 with KOH, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate to the autoclaved medium from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust the pH of the complete medium to 9.8 - 10.0. | |||||
Solution occurs in 12
other media (first defined in Medium J268).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 30.00 | g | 29.703 | 508.264 |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 |
| CaCl2 x 2 H2O | 0.14 | g | 0.139 | 0.943 |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
| MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 |
| KCl | 0.33 | g | 0.327 | 4.383 |
| NiCl2 x 6 H2O | 0.50 | mg | 5.0e-4 | 0.004 |
| Na2SeO3 x 5 H2O | 0.50 | mg | 5.0e-4 | 0.002 |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - |
| Modified Wolin's mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 166
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Metadata
| Last modified: | 18.03.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.8 - 10.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Alkaliphilus transvaalensis DSM 29504
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 2.5 | 29.759 |
| Na2S2O3 x 5 H2O | 2 | 8.058 |
| Yeast extract | 2 | - |
| Tryptone | 2 | - |
| Na2CO3 | 1.25 | 11.794 |
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