J268
268 .
MJ/YPGS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J268 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
1.00 | g | 1 | - | |
|
Bacto peptone
(BD-Difco) |
1.00 | g | 1 | - | |
| Glucose | 0.20 | g | 0.2 | 1.11 | |
| Sodium succinate | 0.20 | g | 0.2 | 1.234 | |
| Na2SiO3 x 9 H2O | 1.00 | g | 1 | - | |
| Na2S2O3 x 5 H2O | 2.48 | g | 2.48 | 9.993 | |
| MJ(-N) synthetic seawater | 100.00 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Adjust pH to 7.5. | |||||
Solution occurs in 12
other media (first defined in Medium J268).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 30.00 | g | 29.703 | 508.264 |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 |
| CaCl2 x 2 H2O | 0.14 | g | 0.139 | 0.943 |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
| MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 |
| KCl | 0.33 | g | 0.327 | 4.383 |
| NiCl2 x 6 H2O | 0.50 | mg | 5.0e-4 | 0.004 |
| Na2SeO3 x 5 H2O | 0.50 | mg | 5.0e-4 | 0.002 |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - |
| Modified Wolin's mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 2.971 | 50.843 |
| Na2S2O3 x 5 H2O | 2.48 | 9.993 |
| Yeast extract | 1 | - |
| Bacto peptone | 1 | - |
| Na2SiO3 x 9 H2O | 1 | - |
| Show more | ||
Cultivation metadata from 1 strains
| phyla | 1 | 1 strain |