1542 .
CY/H LIQUID MEDIUM FOR MYXOBACTERIA
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Equipment needed:
Autoclave
Filter
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 1000 | ml | ||
|
|
Solution B | 1 | ml | ||
|
|
Solution C | 1 | ml | ||
| 1 Adjust pH of solution A to pH 7.4. Autoclave solutions A and B separately. Filter sterilize vitamin solution. After cooling, add 1 ml solution B and 1 ml solution C per liter solution A. | |||||
Solution A #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Casitone | 1.5 | g |
|
|
Yeast extract | 1.5 | g |
|
|
Starch | 4.0 | g |
|
|
Soy flour , defatted | 1.0 | g |
|
|
D-Glucose | 1.0 | g |
|
|
CaCl2 x 2 H2O | 1.0 | g |
|
|
MgSO4 x 7 H2O | 0.5 | g |
|
|
HEPES | 11.9 | g |
|
|
Distilled water | 1000.0 | ml |