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DSMZ

DSMZ 1538 . ANAEROBACTERIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1538 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.2 1.47
NH4Cl 0.25 g 0.25 4.674
NaCl 1.00 g 1 17.112
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KCl 0.50 g 0.5 6.707
CaCl2 x 2 H2O 0.15 g 0.15 1.02
Yeast extract 1.00 g 1 -
Cellobiose 10.00 g 10 29.214
NaHCO3 2.52 g 2.52 29.998
Na2S x 9 H2O 0.36 g 0.36 1.499
Trace element solution SL-10 1.00 ml - -
Vitamin solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Distilled water 1000.00 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 267 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg/l 0.02 0.082
p-Aminobenzoic acid 80 mg 0.08 0.583
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Vitamin B12 50 mg 0.05 0.037
Distilled water 1000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 180 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 20.12.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cellobiose 10 29.214
NaHCO3 2.52 29.998
NaCl 1 17.112
Yeast extract 1 -
KCl 0.5 6.707
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Cultivation metadata from 1 strains BacDive

pH 8 - 10 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
temperature psychrophilic 1 strain