150
150 .
ACIDIANUS BRIERLEYI MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidianus brierleyi DSM 1651
This strain uses the standard medium without modifications.
Growth conditions: 70 °C
Main sol. 150 #
ml
Equipment needed: Autoclave
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 3.00 | g | 3 | 22.703 | |
| K2HPO4 x 3 H2O | 0.50 | g | 0.5 | 2.871 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| Ca(NO3)2 x 4 H2O | 0.02 | g | 0.02 | 0.085 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
|
Sulfur
(powdered) |
10.00 | g | 10 | 311.818 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except yeast extract and sulfur. Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. For sterilization of the sulfur place it in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium. | |||||
Metadata
| Last modified: | 24.07.23 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 1.5 - 2.5 |
| Equipment needed: | Autoclave, Water bath |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Acidianus brierleyi DSM 1651
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 10 | 311.818 |
| (NH4)2SO4 | 3 | 22.703 |
| K2HPO4 x 3 H2O | 0.5 | 2.871 |
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| Yeast extract | 0.2 | - |
| Show more | ||
