# 150: ACIDIANUS BRIERLEYI MEDIUM


## Main sol. 150

3	g	(NH4)2SO4	
0.5	g	K2HPO4 x 3 H2O	
0.5	g	MgSO4 x 7 H2O	
0.1	g	KCl	
0.02	g	Ca(NO3)2 x 4 H2O	
0.2	g	Yeast extract	
10	g	Sulfur (powdered)	
1000	ml	Distilled water	

1. Dissolve ingredients except yeast extract and sulfur. Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. For sterilization of the sulfur place it in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium.