1463
1463 .
YE/10 + Ca MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1463 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(Difco) |
0.30 | g | 0.3 | - | |
| Peptone | 0.06 | g | 0.06 | - | |
| Tris-HCl buffer | 0.61 | g | 0.61 | - | |
| CaCl2 x 2 H2O | 0.30 | g | 0.3 | 2.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add all the components together, except calcium chloride which is added from a sterilized stock solution when the autoclaved medium has cooled. Adjust final pH to 7.5. | |||||
| 2 Propagation of DSM 25223 in liquid cultures: Add 3 ml of a Caulobacter vibrioides DSM 25117 culture (grown for 24 h in DSMZ medium 1462) to 20 ml of medium and inoculate with 1 ml of a liquid culture of DSM 25223 or transfer some material with an inoculation loop from the lysis zone of a double layer agar plate. Incubate with shaking (ca. 150 rpm) for approximately 48 h. | |||||
Metadata
| Last modified: | 18.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tris-HCl buffer | 0.61 | - |
| CaCl2 x 2 H2O | 0.3 | 2.041 |
| Yeast extract | 0.3 | - |
| Peptone | 0.06 | - |
| Distilled water | - | - |
