1462
1462 .
CAULOBACTER PYE MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Caulobacter vibrioides DSM 25117
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 1462 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 2.00 | g | 2 | - | |
|
Yeast extract
(Difco) |
1.00 | g | 1 | - | |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
|
Agar
, for solid medium
optional |
12.00 | g | 12 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add all the components together, except calcium chloride which is added from a sterilized stock solution when the autoclaved medium has cooled. Agar is added at a concentration of 12 g/l for solid media. For agar media, do not add supplemental calcium. | |||||
| 2 Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 04.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Caulobacter vibrioides DSM 25117
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
|
Agar optional |
12 | - |
| Peptone | 2 | - |
| Yeast extract | 1 | - |
| CaCl2 x 2 H2O | 0.07 | 0.476 |
| Distilled water | - | - |
