143
143 .
CLOSTRIDIUM SP. LA1 MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 143 #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Crotonic acid | 6.00 | g | 6 | 69.695 | |
| NaOH | 0.30 | g | 0.3 | 7.501 | |
| (NH4)2HPO4 | 0.15 | g | 0.15 | 1.136 | |
| K2HPO4 | 0.10 | g | 0.1 | 0.574 | |
| MgCl2 x 6 H2O | 0.03 | g | 0.03 | 0.148 | |
| CaCl2 x 2 H2O | 0.04 | g | 0.04 | 0.272 | |
| NH4Cl | 0.05 | g | 0.05 | 0.935 | |
|
MgSO4 x 7 H2O
(0.1% w/v) |
0.60 | ml | 6.0e-4 | 0.002 | |
|
MnSO4 x 2 H2O
(0.1% w/v) |
0.40 | ml | 4.0e-4 | 0.002 | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
0.40 | ml | 4.0e-4 | 0.001 | |
| (NH4)6Mo7O24 x 4 H2O | 10.00 | mg | 0.01 | - | |
|
Biotin
(0.1% w/v) |
0.04 | ml | 4.0e-5 | 1.6e-4 | |
|
p-Aminobenzoic acid
(0.1% w/v) |
0.80 | ml | 8.0e-4 | 0.006 | |
|
Tryptone
(BD BBL) |
1.00 | g | 1 | 14.069 | |
|
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| K2CO3 | 4.00 | g | 4 | 28.942 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except potassium carbonate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. An anoxic stock solution of the potassium carbonate is autoclaved separately under 80% N2 and 20% CO2 gas mixture and thereafter added to the sterile medium. Adjust pH of the complete medium to 6.8 - 7.0. Prior to inoculation add 10 - 20 mg/l sodium dithionite from a 5% (w/v) stock solution that has been freshly prepared under 100% N2 gas and sterilized by filtration to reduce the medium. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Crotonic acid | 6 | 69.695 |
| K2CO3 | 4 | 28.942 |
| Tryptone | 1 | 14.069 |
| Yeast extract | 1 | - |
| NaOH | 0.3 | 7.501 |
| Show more | ||
