MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 142 . THIOMICROSPIRA PELOPHILA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Thiomicrorhabdus frisia DSM 12351

* Modification: Omit vitamins and adjust pH of complete medium to 7.3 - 7.6.

Growth conditions: 30 °C

Main sol. 142 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.00 g 24.876 425.661
(NH4)2SO4 1.00 g 0.995 7.53
MgSO4 x 7 H2O 1.50 g 1.493 6.056
CaCl2 x 2 H2O 0.42 g 0.418 2.843
Trace element solution (Vishniac & Santer, 1957) 0.20 ml - -
Bromothymol blue
(0.1% w/v) 		4.00 ml 0.004 0.006
K2HPO4 0.50 g 0.498 2.856
Na2S2O3 x 5 H2O 5.00 g 4.975 20.046
Seven vitamins solution* 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 7.2 and autoclave. K2HPO4 and Na2S2O3 are autoclaved separately each in 10% of the final volume. Filter sterilize the vitamins solution. Adjust pH of the complete medium to 7.2 with sterile 0.4% (w/v) Na2CO3 solution. Acidification of the medium during growth causes the pH indicator bromothymol blue to turn from blue to yellow.
2 Note: Growth of most Thiomicrospira strains is more reliable if the medium is prepared under a 80% N2 and 20% CO2 gas atmosphere to make it anoxic and then filled under air atmosphere into Hungate-type tubes (5 ml per vial). The pH is adjusted with a sterile stock solution of Na2CO3 (5% w/v) after autoclaving.

Trace element solution (Vishniac & Santer, 1957) #

1000
Solution occurs in 4 other media (first defined in Medium 69).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 05.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: * 7.3 - 7.6
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
Edit in Medium builder

Gas composition

  • 75 % N2
  • 15 % CO2
  • 10 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 19
Strains with modifications: 10
Thiomicrorhabdus frisia DSM 12351

-  Seven vitamins solution
  7.3 - 7.6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.876 425.661
Na2S2O3 x 5 H2O 4.975 20.046
MgSO4 x 7 H2O 1.493 6.056
(NH4)2SO4 0.995 7.53
K2HPO4 0.498 2.856
Show more
CSV
JSON

Cultivation metadata from 19 strains BacDive

pH 6 - 8 3 strains
pH 4 - 6 3 strains
pH 8 - 10 2 strains
phyla 1 19 strains
nutrition chemolithoautotroph 3 strains
Show more