142a
142a .
THIOMICRORHABDUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 142a #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.00 | g | 15 | 256.674 | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| MgSO4 x 7 H2O | 1.50 | g | 1.5 | 6.086 | |
| CaCl2 x 2 H2O | 0.42 | g | 0.42 | 2.857 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Bromothymol blue
(0.1% w/v) |
4.00 | ml | 0.004 | 0.006 | |
| K2HPO4 | 0.50 | g | 0.5 | 2.871 | |
| Na2S2O3 x 5 H2O | 5.00 | g | 5 | 20.146 | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust pH of the complete medium to 7.6 with a sterile 5% (w/v) Na2CO3 solution. | |||||
Solution occurs in 267
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 77
other media (first defined in Medium 503).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 100 | mg | 0.1 | 0.074 |
| p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
| D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
| Nicotinic acid | 200 | mg | 0.2 | 1.625 |
| Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
| Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
| Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 02.03.23 |
| Source: | DSMZ |
| Synonyms: | THIOMICROSPIRA PSYCHROPHILA MEDIUM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.6 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 15 | 256.674 |
| Na2S2O3 x 5 H2O | 5 | 20.146 |
| MgSO4 x 7 H2O | 1.5 | 6.086 |
| (NH4)2SO4 | 1 | 7.568 |
| K2HPO4 | 0.5 | 2.871 |
| Show more | ||
