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DSMZ

DSMZ 142a . THIOMICRORHABDUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 142a #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.00 g 15 256.674
(NH4)2SO4 1.00 g 1 7.568
MgSO4 x 7 H2O 1.50 g 1.5 6.086
CaCl2 x 2 H2O 0.42 g 0.42 2.857
Trace element solution SL-10 1.00 ml - -
Bromothymol blue
(0.1% w/v) 		4.00 ml 0.004 0.006
K2HPO4 0.50 g 0.5 2.871
Na2S2O3 x 5 H2O 5.00 g 5 20.146
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust pH of the complete medium to 7.6 with a sterile 5% (w/v) Na2CO3 solution.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 02.03.23
Source: DSMZ
Synonyms: THIOMICROSPIRA PSYCHROPHILA MEDIUM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.6
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 4
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
Na2S2O3 x 5 H2O 5 20.146
MgSO4 x 7 H2O 1.5 6.086
(NH4)2SO4 1 7.568
K2HPO4 0.5 2.871
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Cultivation metadata from 4 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 2 strains
phyla 2 4 strains
nutrition chemolithoautotroph 2 strains
oxygen aerobe 2 strains
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