1372 .
SUCCINATE MINIMAL SALTS MEDIUM (SMS)
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Equipment needed:
Autoclave
Filter
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 0.010 | g | ||
|
|
KH2PO4 | 0.600 | g | ||
|
|
K2HPO4 | 0.900 | g | ||
|
|
NH4Cl | 1.000 | g | ||
|
|
MgSO4 x 7 H2O | 0.200 | g | ||
|
|
CaCl2 x 6 H2O | 0.075 | g | ||
|
|
Sodium succinate | 2.200 | g | ||
|
|
Yeast extract | 0.100 | g | ||
|
|
Trace elements solution | 2.000 | ml | ||
|
|
Vitamin solution | 2.000 | ml | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Adjust pH to 6.8. | |||||
| 2 Prepare the medium without the vitamin solution. | |||||
| 3 Autoclave and add the vitamin solution from a filter-sterilised stock solution. | |||||
| 4 Incubate strains in liquid culture with air headspace in closed vessels. | |||||
Trace elements solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 6 H2O | 0.3 | g |
|
|
ZnSO4 x 7 H2O | 5.0 | mg |
|
|
MnCl2 x 4 H2O | 3.0 | mg |
|
|
H3BO3 | 2.0 | mg |
|
|
CoCl2 x 6 H2O | 5.0 | mg |
|
|
CuCl2 x 2 H2O | 1.0 | mg |
|
|
NiCl2 x 6 H2O | 2.0 | mg |
|
|
Na2MoO4 x 2 H2O | 3.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 80 | mg |
|
|
Thiamine-HCl x 2 H2O | 400 | mg |
|
|
Nicotinic acid | 400 | mg |
|
|
Vitamin B12 | 20 | mg |
|
|
Distilled water | 1000 | ml |