1326 .
AURANTIMONAS MANGANOXYDANS MEDIUM
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Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.5 | g | ||
|
|
Peptone | 2.0 | g | ||
|
|
Artificial seawater | 980.0 | ml | ||
|
|
HEPES buffer
(1 M) |
20.0 | ml | ||
|
|
Ferric ammonium citrate
(3 mg/ml) |
0.1 | ml | ||
|
|
Agar , for solid medium | 15.0 | g | ||
| 1 After autoclaving add 20ml 1M HEPES sterile buffer (pH 7.8) and 0.1ml of a sterile 3mg/ml of ferric ammonium citrate. Final pH is 7.5. The medium may be solidified by adding 15 g/l agar. | |||||
Artificial seawater #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 17.55 | g |
|
|
KCl | 0.75 | g |
|
|
MgSO4 x 7 H2O | 12.35 | g |
|
|
CaCl2 x 7 H2O | 1.46 | g |
|
|
Distilled water | 1000.00 | ml |