# 1326: AURANTIMONAS MANGANOXYDANS MEDIUM


## Main sol. 1326

0.5	g	Yeast extract	
2	g	Peptone	
980	ml	**Artificial seawater**	
20	ml	HEPES buffer (1 M)	
0.1	ml	Ferric ammonium citrate (3 mg/ml)	
15	g	Agar, for solid medium	

1. After autoclaving add 20ml 1M HEPES sterile buffer (pH 7.8) and 0.1ml of a sterile 3mg/ml of ferric ammonium citrate. Final pH is 7.5. The medium may be solidified by adding 15 g/l agar.


## Artificial seawater

17.55	g	NaCl	
0.75	g	KCl	
12.35	g	MgSO4 x 7 H2O	
1.46	g	CaCl2 x 7 H2O	
1000	ml	Distilled water