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DSMZ

DSMZ 1321 . ACIDIHALOBACTER FERROOXIDANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Acidihalobacter yilgarnensis DSM 105917

* Modification: Omit yeast extract and adjust pH of complete medium to 2.5.

Growth conditions: 3-7 days 30 °C

Main sol. 1321 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 100 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Combine solution A and solution B after cooling and add solutions C and D to complete the medium.
2 Note: Use 10% (v/v) as inoculum.

Solution A #

900
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.0 g 16.667 285.193
(NH4)2SO4 0.2 g 0.222 1.682
MgSO4 x 7 H2O 0.4 g 0.444 1.803
K2HPO4 0.1 g 0.111 0.638
Distilled water 900.0 ml - -
1 Adjust the pH to 1.7 with H2SO4 and autoclave at 121°C for 20 min.

Solution B #

100
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 13.9 g 139 499.973
Distilled water 100.0 ml - -
1 Adjust the pH to 1.25 and autoclave solution at 115°C for 10 min or sterilize by filtration.

Solution C #

10
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2S4O6 0.3 g 30 -
Distilled water 10.0 ml - -
1 Sterilize by filtration.

Solution D #

10
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract* 0.1 g 10 -
Distilled water 10.0 ml - -
1 Autoclave at 121°C for 20 min.

Metadata

Last modified: 04.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 2.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2
Strains with modifications: 1
Acidihalobacter yilgarnensis DSM 105917

  2.5
  Yeast extract

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
NaCl 14.706 251.641
FeSO4 x 7 H2O 13.628 49.017
MgSO4 x 7 H2O 0.392 1.591
K2S4O6 0.294 -
(NH4)2SO4 0.196 1.484
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Cultivation metadata from 2 strains BacDive

halophily halotolerant 1 strain
pH 0 - 2 1 strain
pH 2 - 4 1 strain
phyla 1 2 strains
nutrition chemolithoautotroph 1 strain
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