1321 .
ACIDIHALOBACTER FERROOXIDANS MEDIUM
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* Medium for Acidihalobacter yilgarnensis DSM 105917
Omit yeast extract and adjust pH of complete medium to 2.5.
Growth conditions: 3-7 days
30 °C
Equipment needed:
Autoclave
Filter
Final pH:
* 2.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 900 | ml | ||
|
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Solution B | 100 | ml | ||
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Solution C | 10 | ml | ||
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Solution D | 10 | ml | ||
| 1 Combine solution A and solution B after cooling and add solutions C and D to complete the medium. | |||||
| 2 Note: Use 10% (v/v) as inoculum. | |||||
Solution A #
900
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 15.0 | g | ||
|
|
(NH4)2SO4 | 0.2 | g | ||
|
|
MgSO4 x 7 H2O | 0.4 | g | ||
|
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K2HPO4 | 0.1 | g | ||
|
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Distilled water | 900.0 | ml | ||
| 1 Adjust the pH to 1.7 with H2SO4 and autoclave at 121°C for 20 min. | |||||
Solution B #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeSO4 x 7 H2O | 13.9 | g | ||
|
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Distilled water | 100.0 | ml | ||
| 1 Adjust the pH to 1.25 and autoclave solution at 115°C for 10 min or sterilize by filtration. | |||||
Solution C #
10
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2S4O6 | 0.3 | g | ||
|
|
Distilled water | 10.0 | ml | ||
| 1 Sterilize by filtration. | |||||
Solution D #
10
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
| Yeast extract* | 0.1 | g | |||
|
|
Distilled water | 10.0 | ml | ||
| 1 Autoclave at 121°C for 20 min. | |||||